Identify vector integration site in a efficient way
Assuming we suspect that a retrovirus or artificial vector integrated into human genome, how can we find out the exact chromosome location of the integration using NGS based method? First we need have our vector containing sample sequenced. If we have an estimation of where the integration sites are, we can go with targeted sequencing that only focuses on the region of interest. However, In most cases we don't have this knowledge in advance and have to sequence entire human g