DNA Ghost:

This note based blog covers bioinformatics and cancer genomics 

November 29, 2017

Assuming we suspect that a retrovirus or artificial vector integrated into human genome, how can we find out the exact chromosome location of the integration using NGS based method? 

First we need have our vector containing sample sequenced. If we have an esti...

November 25, 2017

Note that the statistics we are going to talk about only applies to germline mutation, not somatic mutation.

Once we called a list of SNPs. it is routine that we get some statistics out of them. These statistics commonly include:

Transition / Transversion (Ti / Tv) rati...

November 23, 2017

Earlier I started a discussion regarding Duplicates issue in NGS. I later had some further thoughts about this topic and would like to mention it here. It is maybe trivial, but still interesting to think about it.

In the early article I mentioned the source of PCR dupli...

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