DNA Ghost:

This note based blog covers bioinformatics and cancer genomics 

December 11, 2017

During the standard library preparation, we usually use physical or enzymatic methods to fragment DNA. How different fragment size affects downstream analysis and how do we choose optimal fragment size? 

Generally speaking, the optimal fragment size depends on the goal...

December 10, 2017

Copy number variations (CNVs) have been well studied regarding their association with tumorigenesis. Meanwhile, numerous targeted therapies have been approved to target specific CNV harbored in the tumor cells. Here we talk about some basics of CNV and its detection....

December 1, 2017

bam file may store more alignment information than you think. As probably the most important format in NGS analysis, almost all sorts of variants are identified from it. It is critical that we get a full image of bam format.

Here I like to mention some of the subtle tag...

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