MCMC II: Applying MCMC in somatic variant callingIn PREVIOUS ARTICLE, we introduced theoretical basis of MCMC, its components and how it approximates the posterior distribution of...
MCMC: Monte Carlo sampling and Markov ChainIn PREVIOUS ARTICLE, we mentioned that Bayesian approach is being used for somatic variant detection. We will pick up this topic from...
Probability distribution in NGS data analysisIn previous POST, we discussed some basics of probability distribution and corresponding hypothesis testing. Here let's continue this...
Calling somatic variants without matched normalIdentifying somatic mutation has always been challenging. Tumor heterogeneity The major challenge of calling somatic mutation is...
From variant calling approach: Frequentist vs BayesianFrequentist's approach: Assume sequencing error distribution. Frequentist's method makes the null hypothesis that non-reference allele is...