For RNA-Seq, it has been challenging to distinguish PCR duplicates from biological duplicates. Ghost found a tool that tackles this and answer question: In your RNA-Seq library, whether the duplicates primarily come from PCR duplicates or biological duplicates? Does your RNA-Seq library has enough complexity (DNA material) to continue expression analysis? Why is duplicates problematic in RNA-Seq? Certain level of duplicates is unavoidable in RNA-Seq. It has been a lasting arg